pancreatic regeneration  (BioVendor Instruments)

Journal: Frontiers in Medicine

Article Title: The critical role of pancreatic stone protein/regenerating protein in sepsis-related multiorgan failure

doi: 10.3389/fmed.2023.1172529

Figure Lengend Snippet: Correlation between circulating Pancreatic stone protein/regenerating protein (PSP/Reg) levels and prognosis as well as progression to multiple organ dysfunction syndrome (MODS) in patients with sepsis admitted to an intensive care unit. (A) Circulating PSP/Reg levels were compared according to disease severity in patients. Mann–Whitney U test showed a significant increase in PSP/Reg levels in patients with septic shock compared with an increase in those with severe sepsis ( p < 0.001). (B) PSP/Reg levels were evaluated in patients stratified by Sequential Organ Failure Assessment (SOFA) scores, and Kruskal–Wallis test showed a significant increase in PSP/Reg levels (median and interquartile range) with increasing SOFA scores ( p < 0.001). Mann–Whitney U test indicated significant differences in PSP/Reg levels between patients with and without MODS (C) and between survivors and non-survivors of sepsis. (D) * p < 0.05, ** p < 0.01. (E) According to the quartile division of PSP/Reg at admission, the Kaplan–Meier survival curve for 28 days is shown. The receiver operating characteristic curve analysis of PSP/Reg for the prediction of MODS (F) and 28d mortality (G) .

Article Snippet: Recombinant pancreatic stone protein/regenerating protein (PSP/Reg) (Sino Biological) was dissolved in PBS of 100 μL, and the solution was injected into the caudal vein 30 min after CLP at 40 and 400 ng/kg.

Techniques: MANN-WHITNEY

Journal: Frontiers in Medicine

Article Title: The critical role of pancreatic stone protein/regenerating protein in sepsis-related multiorgan failure

doi: 10.3389/fmed.2023.1172529

Figure Lengend Snippet: Pancreatic stone protein/regenerating protein (PSP/Reg) exacerbates multiple organ failure in a cecal ligation and puncture (CLP) mouse model. Mice were randomly selected to undergo CLP surgery and were intravenously administered with phosphate-buffered saline (PBS), PSP/Reg (40 ng/kg), or PSP/Reg (400 ng/kg) 30 min later. (A) Murine sepsis score at 24 h after CLP was significantly increased by PSP/Reg treatment by one-way analysis of variance (ANOVA). (B) Survival curves and log-rank test showed that compared with PBS, PSP/Reg significantly shortened the survival time of the mice. Serum levels of inflammatory cytokines and organ-damage markers were evaluated at 24 h after CLP. (C) tumor necrosis factor-α levels (ng/mL), (D) interleukin (IL)-6 levels (pg/mL), (E) IL-1β levels (pg/mL), (F) lactate dehydrogenase (U/L), (G) troponin I (ng/mL), (H) creatinine (mg/dL), and (I) lung wet/dry (W/D) ratio were measured. Data are expressed as the mean ± standard error of the mean and analyzed by one-way ANOVA. * p < 0.01, ** p < 0.001, compared with the PBS group.

Article Snippet: Recombinant pancreatic stone protein/regenerating protein (PSP/Reg) (Sino Biological) was dissolved in PBS of 100 μL, and the solution was injected into the caudal vein 30 min after CLP at 40 and 400 ng/kg.

Techniques: Ligation

Journal: Frontiers in Medicine

Article Title: The critical role of pancreatic stone protein/regenerating protein in sepsis-related multiorgan failure

doi: 10.3389/fmed.2023.1172529

Figure Lengend Snippet: Pancreatic stone protein/regenerating protein (PSP/Reg) increased neutrophil infiltration in multiple organs of cecal ligation and puncture (CLP) mice. The level of myeloperoxidase (MPO) activity and neutrophil (Ly6G+) infiltration in multiple organ tissues were measured 24 h after CLP in mice treated with PSP/Reg. (A) : lung tissue MPO activity, (B) : kidney tissue MPO activity, (C) : heart tissue MPO activity, and (D) : liver tissue MPO activity. The data were analyzed by one-way analysis of variance. * p < 0.01, ** p < 0.001 compared with the PBS group. Representative images of Ly6G+ (green fluorescence) areas in lung (E) , kidney (F) , heart (G) , and liver (H) tissues. Scale bar, 20 mm.

Article Snippet: Recombinant pancreatic stone protein/regenerating protein (PSP/Reg) (Sino Biological) was dissolved in PBS of 100 μL, and the solution was injected into the caudal vein 30 min after CLP at 40 and 400 ng/kg.

Techniques: Ligation, Activity Assay, Fluorescence

Journal: Frontiers in Medicine

Article Title: The critical role of pancreatic stone protein/regenerating protein in sepsis-related multiorgan failure

doi: 10.3389/fmed.2023.1172529

Figure Lengend Snippet: Pancreatic stone protein/regenerating protein (PSP/Reg) increased the levels of neutrophil surface adhesion molecules. Cecal ligation and puncture mice were stimulated with PSP/Reg 30 min after surgery, and 24 h later, flow cytometry was performed to detect the percentage of intercellular adhesion molecule 1 (ICAM-1)-positive (A) and CD29-positive (B) cells in mouse bone marrow neutrophils. Neutrophils were isolated from the mouse bone marrow and stimulated with 40 ng/ml and 400 ng/ml of PSP/Reg overnight. (C) Representative histograms and MFI quantification analysis of ICAM-1 levels in PMN under 40 ng/ml stimulation. (D) Representative histograms and MFI quantification analysis of CD29 levels in PMN under 400 ng/ml stimulation.The data were analyzed by one-way analysis of variance. * p < 0.01, ** p < 0.001 compared with the PBS group.

Article Snippet: Recombinant pancreatic stone protein/regenerating protein (PSP/Reg) (Sino Biological) was dissolved in PBS of 100 μL, and the solution was injected into the caudal vein 30 min after CLP at 40 and 400 ng/kg.

Techniques: Ligation, Flow Cytometry, Isolation